Dear Debbra Marcel, according to our experience, not always. But, there are chances for slight surface morphological changes to occur during their presence in soil for very long time.
Thank you Mr. Rakesh. I agree with you. My current study is detecting the presence of Toxocara eggs from soil samples. I found out many of the Toxocara eggs (I suppose) covered with dirts and some even hard to see the eggs structure clearly. The clear image of Toxocara eggs mainly found in dogs very seldom to find. Can you share any literature saying about this?
Dear Debbra Marcel, T. canis eggs excreted into the environment with dogs feces are unembryonated. They usually become embryonated within two weeks.
You can examine the soil samples by zinc sulphate flotation method ,one part soil will be homogenized in 10 parts tap water filtered through a gauze to remove coarse debris and centrifuged at 2000 rpm for 5 minutes .the supernatant will be poured away and the process repeated twice. Then, 10 parts zinc sulphate solution will be added to one part pellet, the tube will be shaken , allowed to stand for 30 minutes , and triplicate samples from the surface will be removed and examined on glass microscopic slides.