For example, if I have a ~3000 nt species I want to purify, should I use the ratios recommended for a 3000bp species? Or for a 1500bp species, since it's single stranded?
I've recently run into serious problems with my Western blot protein transfers to NC membrane. When I do the Ponceau stain, I get something as shown in the picture below - white blotches covering...
15 December 2018 2,554 6 View
I want to inhibit Ser/Thr phosphatase activity in synchronized live cells, and I would like to find out if/how compounds like NaF, Na orthovanadate, or beta glycerol phosphate can be used in live...
07 November 2018 9,028 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View
Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...
14 July 2024 7,475 1 View
Hi all, I am conducting research on screening peptides (20-35 amino acids) with potential for treating dengue fever through protein-peptide docking methods. In the protein database, I found the...
10 July 2024 8,037 1 View