Nomi Pernicone

3 Questions 4 Answers 0 Followers

Questions related from Nomi Pernicone

Are the bead:sample ratios for SPRI beads the same for single stranded nucleic acids as for double stranded?

For example, if I have a ~3000 nt species I want to purify, should I use the ratios recommended for a 3000bp species? Or for a 1500bp species, since it's single stranded?

09 May 2023 183 0 View

Ponceau staining/transfer problem?

I've recently run into serious problems with my Western blot protein transfers to NC membrane. When I do the Ponceau stain, I get something as shown in the picture below - white blotches covering...

16 December 2018 2,538 6 View

Ser/Thr phosphatase inhibitor - for live cell culture?

I want to inhibit Ser/Thr phosphatase activity in synchronized live cells, and I would like to find out if/how compounds like NaF, Na orthovanadate, or beta glycerol phosphate can be used in live...

08 November 2018 9,012 1 View