Are short-chained fatty acids good for mAb secretion?

More Dongdong Hu's questions See All

How to eliminate imaginary phonon frequency in phonon dispersion of Coved Graphene Nanoribbons?

Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...

17 June 2024 6,911 2 View

Mutagenesis question, 9 mismatch pcr cycle?

Dear all, I am currently trying to perform mutagenesis to change 9 mismatch. Is it possible to do it in one round of PCR? if so, are there any protocol I can learn from?

16 June 2024 875 2 View

Is it correct to call model 2 (Hayes Process) a double moderation model?

I am using PROCESS (model 2) to conduct moderation analysis with two moderators (moderator W is a categorical variable while moderator Z is a continuous variable). DV is predicted by X (IV), W, Z,...

07 May 2024 8,042 0 View

Zebra Finch Craniotomy?

Where is the HVC beside the letter Y in Zebra Finch's brain craniotomy?

25 February 2024 2,623 0 View

How to autoclave micro centrifuge tube?

Hi, I need to autoclave my sample (only 400ul) in micro centrifuge tube. Does anyone know how to autoclave it? Thanks!

22 February 2024 9,216 0 View

What are the similarities and differences between radiation and irradiation?

There is some confusion in the use of these two words.

05 February 2024 7,110 5 View

How to investigate the mechinism of transcrition factor and genes?

Hi, I have fond a strong negative correlation between a transcription factor and genes. How could I further decipher the underling mechanism? Thank you

23 January 2024 7,056 8 View

How to separate dead C.elegans from living ones?

The dead and alive C.elegans is mixed on one NGM plate, how can I separate alive one from dead one in a very rapid way? Besides , those living C.elegans has very low athletic ability.

10 January 2024 4,484 1 View

Does KEGG or MetaCyc provide a batch search or bulk search feature?

Hi! Now I have 500 enzyme ECs and I want to figure out which pathway each EC belongs to. One option is that I can click the search button 500 times, but I'm wondering if there's any batch query...

26 December 2023 3,722 0 View

How to get better XRR simulation of PS film on Si substrate?

Dear Colleaguse, We are trying to simulate the XRR nanolayers to get information about the density parameters of PS films on Si substrate. And I found that no matter how I adjusted the parameters...

22 December 2023 7,038 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Zahira Abduljabbar Abdulameer Al-Zuhairi

www.fibercellsystems.com/

Fai Poon

Cells have more ER, Gogi bodies etc for packing the target protein， hence higher productivity. I would stain these organelles to prove this hypothesis. Also, bigger cells produce more protein. This observation also fits the hypothesis.