I have been measuring the metabolic rates (O2 consumed and CO2 produced) in bobwhite quail embryos at different temperatures. The average RQ is about .51.
How is this possible? I thought RQ should range between .7 and 1.
I don`t know exactly about the bird embryo, but one of paper that I read mentioned that consumption and CO2 production could only measured after day 12 of incubation because concentrations of these gases are very small during the early periods of incubation. But they did it on chicken embryo, hopefully it will work for you
If there is any carbon dioxide retention taking place, subsequent to perhaps some acid accumulation, there can be low RQs. In goldish, low RQs were observed during recovery phase after a hypoxic exposure.