It seems that resazurin-based viability assays (such as Alamar Blue) are frequently used with hepatocytes or hepatic cell lines cultures, but the effect of resaruzin on the induction of cytochroms P450 on the samples is barely discussed. As these tests are usually non-destructive, it is appealing to use the same sample for the viability assay then CYP induction analysis, but I can't find an actual proof that resazurin won't affect the CYP activity. Does anyone have feedback or insight on this question? Thanks a lot!
Hi Timothée,
Incubation with resazurin definitely affects the parameters of cells. Viability reestimated in 24 or 48 hr was lower than in cells treated in the same manner but without resazurin. Moreover, it sensitizes HaCaT cells to following exposure to UV-B.
Taking into account the structure of resazurin it should somehow affect the detoxication system. But I still have no proves.
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