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Questions related from Freyda Lenihan-Geels
I have tried purifying a fluorescently-labelled (Alexa647) PCR product from agarose gel using the Macherey Nagel Nucleospin kit but even after several elution attempts (reapplying the eluted...
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I am running an EMSA with a myogenic TF (myogenin with N-terminal GST tag expressed in BL21 E coli) and its wildtype binding site (labelled with Alexa647 for visualisation). I use 100 fmol...
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I am attempting to perform an EMSA with a transcription factor and its wildtype binding sequence but the first attempt showed that the protein never left the well. After some research, I have...
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