Freyda Lenihan-Geels

4 Questions 7 Answers 0 Followers

Questions related from Freyda Lenihan-Geels

Are native precast gradient gels good for EMSAs?

I am wanting to switch from self-cast to pre-cast gels for my EMSAs. I am currently trying to decide whether to buy a constant percentage gel (eg. 6%) native gel or whether to try a 4-16% gel for...

04 March 2024 7,914 0 View

How can fluorescently-labelled PCR products be purified from agarose gel?

I have tried purifying a fluorescently-labelled (Alexa647) PCR product from agarose gel using the Macherey Nagel Nucleospin kit but even after several elution attempts (reapplying the eluted...

06 October 2023 9,758 3 View

EMSA shift stuck in wells?

I am running an EMSA with a myogenic TF (myogenin with N-terminal GST tag expressed in BL21 E coli) and its wildtype binding site (labelled with Alexa647 for visualisation). I use 100 fmol...

31 October 2022 8,241 1 View

How should you run an EMSA gel for a basic protein?

I am attempting to perform an EMSA with a transcription factor and its wildtype binding sequence but the first attempt showed that the protein never left the well. After some research, I have...

11 February 2022 8,385 4 View