Mupirocin is antibacterial and morin hydrate is anti - inflammatory in nature as far as mechanism of action of both drugs have been concerned? but i want to develop simultaneous HPLC method of both drugs. I did literature review regarding the same and implemented the same for the development of the method. but, none of the peaks were obtained in of the mobile phase. I sincerely request you to suggest valuable information. I tried the mobile phase like methanol + phhosphate buffer/ammonium acetate/sodium acetate buffer/ (20 mM/50 mM, pH = 3,5,7, 70:30 ratio).