Are melanoma A101D cells easily transfected or lentivirus infected?

28 November 2024 1 762 Report

Tried transfection of a GFP plasmid in A101D cells with Lipofectamine 2000, Lipofectamine 3000, XTreme, the efficiency was not statisfied, less than 1% cells were GFP positive. Also tried electroporation, not sucessful. Lentivrus induction is ongoing now, but didn't show encouraging results. Does anyone have experience in A101D transfection or lentivirus induction? Please share your experience here. Thanks!

Naveen Raj

Try to find the titre of lentivirus and infect at a higher MOI. Also infect with centrifugation based method along with polybrene

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