Naveen Raj

3 Questions 2 Answers 0 Followers

Questions related from Naveen Raj

DNA transfection reagent for viral transduction ?

Is it feasible to employ a DNA transfection reagents such as Lipofectamine, SuperFect, or JetPEI to enhance the efficiency of viral transduction? Is there a coherent rationale supporting this...

13 October 2023 4,113 0 View

Can I use a sequence which is optimized for E. coli to express the same in mammalian cells ?

I am having a set of DNA which is the optimized for its expression in E. coli. Can I use the same set of DNA sequence to express it in mammalian cell lines?

30 April 2023 7,986 4 View

Can a protein able to be there without the start codon methionine?

I have extracted a protein sequence from genbank whose starting amino acid is not methionine. Is it feasible for translation? if we need to construct the same in a vector what can we do?

10 January 2023 2,188 2 View