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Does anyone have experience with MPRA library construction using degenerate barcodes? We constructed a library containing ~2,000 oligos, each tagged with a 16N degenerate barcode. After performing...
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Tried transfection of a GFP plasmid in A101D cells with Lipofectamine 2000, Lipofectamine 3000, XTreme, the efficiency was not statisfied, less than 1% cells were GFP positive. Also tried...
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We are trying to set up a doxycycline inducible coding gene expression system in leukemia cells. We tested a plasmid which has a minimal CMV promoter for coding gene expression. it seems that the...
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