Are LysM-cre mice deleted in dendritic cells?

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How should I adjust my HPLC method for a similar column with different dimensions?

I am trying to run a method from a column that is 250x4.6 mm, 8 um particle size into a column that is 300x7.8 mm, 9 um particle size. The makeup of the column is pretty much the same. They are...

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Trouble detecting organic acids with LC-RI detector?

I am trying to detect organic acids on my waters LC, which is equipped with a W410 refractor index detector. I am using a Aminex HPX-87H Column which is supposed to work to separate organic acids....

26 April 2024 3,036 1 View

MaxQuant is throwing a configuring error, can someone help me figure out what it means?

I am running MaxQuant and it starts running and almost immediately stops. I go into the error folder (combined-->proc, then select Configuring 11.error). The screenshot of the error is...

14 February 2024 6,348 1 View

Mixture neutralization after Boc deprotection ?

I am performing a Boc-deprotection with HCl in organic solvent (dioxane or Ethylacetate) but would like to isolate the free amine. Several problems: - Neutralizing with inorganic base as NEt3...

18 February 2021 3,707 3 View

Pure dendritic cells from BMDC ?

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12 January 2021 5,328 2 View

Do I have to include the transcript of the focus group in my appendices?

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21 December 2019 4,014 4 View

Can I mix DNA extracts from the same donor but obtain with different methods extraction kit for make a whole genome sequencing?

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17 June 2019 4,506 0 View

Chip-seq NF-kB in human macrophages?

Hello, I am looking for ChIP-seq data for NF-kB in human monocytes or macrophages. Are you aware of such results? I am so far unable to find them. Thank you very much in advance. Germain

11 December 2017 2,042 2 View

Murine P2X7 western blot?

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How to define fronts position (of surface analysis maps)?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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Detaching human-derived monocytes?

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Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

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Why not use CD11b staining to evaluate the purity of primary microglia?

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Is it hard to stain CCR2 for flow cytometry?

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I want to standardize the human platelet antigen primers for HPA genotyping. What protocol I should follow?

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Tetramer binding not specific for HLA allele???

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Treatment protocol for SRSE - what works best?

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What is the good dilution factor for mouse serum in ELISA?

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Cytokines production in HEK293T cells?

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Joao G Magalhaes

Hi Germain,

yes and no. Some people report no change in the DC population and some yes.

You have to check on your mice but the theory is normally DC are not affected.

cheers.

Germain Rousselet

Thanks Joao!

Am I wrong to say that mo-DC should be deleted? As they differentiate from monocytes, which express lysM, they should be deleted at the monocyte step, should'nt they?