Chip-seq NF-kB in human macrophages?

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How should I adjust my HPLC method for a similar column with different dimensions?

I am trying to run a method from a column that is 250x4.6 mm, 8 um particle size into a column that is 300x7.8 mm, 9 um particle size. The makeup of the column is pretty much the same. They are...

29 April 2024 9,983 1 View

Trouble detecting organic acids with LC-RI detector?

I am trying to detect organic acids on my waters LC, which is equipped with a W410 refractor index detector. I am using a Aminex HPX-87H Column which is supposed to work to separate organic acids....

26 April 2024 3,036 1 View

MaxQuant is throwing a configuring error, can someone help me figure out what it means?

I am running MaxQuant and it starts running and almost immediately stops. I go into the error folder (combined-->proc, then select Configuring 11.error). The screenshot of the error is...

14 February 2024 6,348 1 View

Mixture neutralization after Boc deprotection ?

I am performing a Boc-deprotection with HCl in organic solvent (dioxane or Ethylacetate) but would like to isolate the free amine. Several problems: - Neutralizing with inorganic base as NEt3...

18 February 2021 3,707 3 View

Pure dendritic cells from BMDC ?

I need to obtain dendritic cells that are free of macrophages. After the Helft paper (Immunity, 42:1197, 2015), this seems difficult from BMDCs ? Even sorting out F4/80+ cells should not work,...

12 January 2021 5,328 2 View

Do I have to include the transcript of the focus group in my appendices?

I'm currently pulling together the Appendices for my thesis and I was unclear as to whether the transcript from the focus group should be included or not. I already have all the questions which...

21 December 2019 4,014 4 View

Can I mix DNA extracts from the same donor but obtain with different methods extraction kit for make a whole genome sequencing?

Can I mix DNA extracts from the same donor but obtain with different methods extraction kit for make a whole genome sequencing. The methods are qiagen, whole blood midi kit, zymo mini kit and...

17 June 2019 4,506 0 View

Murine P2X7 western blot?

Hello, I am trying to do western blots of murine P2X7, without success so far. Does anyone know a good antibody for western blotting of P2X7 from murine cells? Thank you very much in advance.

07 December 2017 879 3 View

Are LysM-cre mice deleted in dendritic cells?

It is known that LysM-cre mice are deleted in monocytes, macrophages and neutrophils, but what about dendritic cells?

01 March 2017 293 3 View

How to define fronts position (of surface analysis maps)?

Dear all, I'd like to know how are defined on a surface map the position of fronts (cold and warm) that we find on meteorological surface pressure maps (see link). I want to plot those fronts at a...

02 December 2016 8,932 9 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

Human macrophages cell surface markers?

I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...

29 July 2024 236 2 View

Looking for articles that provide guidance on doing Thematic Analysis (Braun & Clarke) as a team?

Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...

23 July 2024 7,819 5 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Detaching human-derived monocytes?

Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...

20 July 2024 7,922 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

Should HIV testing also be offered to the low prevalence populations?

With many Northern Hemisphere countries approaching or exceeding the 95-95-95 criteria and with improved accessibility of community testing centers, I wonder whether we could significantly...

09 July 2024 2,181 1 View