I have frozen cells (dissociated from mouse tumors) that showed a very low percentage of the live cell population. Even though it had a high number of live cells present, it was too low as a percentage (approx. 20% viable cells). As a result, cells were not processed for the SC-RNA seq and were frozen. I was wondering if I could grow those cells in the plate and then proceed with the SC-RNA seq. Would that be a good option to follow? Or should I use the dead cell removal kit (which was used and showed 200 cells/uL; >70% viable cells) and do SC-RNA seq? I have a lot of such samples to use. Or should I opt for SN-RNA seq? What could be the best idea to go with?
Thank you.
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