As soon as the seeds come out of gamma chamber they are safe to handle. The seeds cannot 'hold' the radiation inside them. The radiation passes through the seeds and induce mutations.
Treatment conditions and doses need to be experimentally derived for new or minor crop species as information may not be readily available. A suitable dose for irradiation has often been recommended as the one that reduces growth and morphogenic performance to 50% (GR 50) of untreated controls, but for seeds it is recommended to use 2–3 doses [19]. The main external factors affecting irradiation are oxygen and moisture contents, temperature and post-irradiation storage conditions.
Thanks for all, these answering not for me only but to one member of the upgrading committee when he evaluate paper to me he said that the plants treated before planing with irradiation may be causing some diseases after eating this plants and i said absolutely non so, i tried to approve that by some one have an expertise in this field.