i grew cancer cells on a transwell insert and then place an agarose cushion over the cells, followed by a weight on top of it. i notice that if the cells are more confluent (100 percent for instance), they survive the compression better. However, if they are about 60-80 percent confluent, they die.
Any reasonings for this.?? is force distributed laterally or do confluent cells have more tight junctions, protein-protein interactions allowing better coping of stress?
Philipp Niethammer
Notably, confluent cell layers are more robust against any kind of stress, mechanical and chemical.
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