C57BL/6 is common for functional examination of BMDC, but why? Also what about other kinds of mice, say like Balb/c, which could yield more BMDC than B6?
I used human bone marrow aspirate to culture MSC, sometimes the sample is mixed with EDTA. I have notice these samples show different proliferation rate and morphology from the the samples without...
10 November 2015 2,563 3 View
Greetings! I have one kind of cell from two patients and I'd like to do a long-term(30 days) co-culture experiment with them. Then I'll have to seperate these two cell for the following...
09 October 2015 7,599 3 View
I'm using 10%FBS RPMI 1640 with 2-Mercaptoethanol, Sodium Pyruvate,HEPES, Non-Essential Amino Acids and Antibiotic-Antimycotic, can I make a mix of all these media supplements prior usage and...
09 October 2013 3,423 3 View
I'm currently using RPMI 1640 (plus 10% FBS, 55 μM 2-mercaptoethanol, 1mM sodium pyruvate,1mM HEPES, 1mM NEAA,1% antibiotic-antimyetic ) for BM-DCs and MLR. Any suggestions to improve this...
09 October 2013 1,967 2 View
Splenocytes were harvested from B6, 1X RBC lysis buffer (eBioscience) was used according to the manufacturer‘s protocol, cell aggregation was easily seen after RBC lysis but their technique...
09 October 2013 338 9 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View