Alexa Wu

6 Questions 5 Answers 0 Followers

Questions related from Alexa Wu

How bad can EDTA influence a MSC culture?

I used human bone marrow aspirate to culture MSC, sometimes the sample is mixed with EDTA. I have notice these samples show different proliferation rate and morphology from the the samples without...

11 November 2015 2,530 3 View

How many ways there are to tell apart the cells of two origins co-cultured together?

Greetings! I have one kind of cell from two patients and I'd like to do a long-term(30 days) co-culture experiment with them. Then I'll have to seperate these two cell for the following...

10 October 2015 7,557 3 View

Are Balb/c mice not suitable for a BMDC study?

C57BL/6 is common for functional examination of BMDC, but why? Also what about other kinds of mice, say like Balb/c, which could yield more BMDC than B6?

05 May 2014 9,938 0 View

Is it right to make a mix of all media supplements before adding to the basic culture media?

I'm using 10%FBS RPMI 1640 with 2-Mercaptoethanol, Sodium Pyruvate,HEPES, Non-Essential Amino Acids and Antibiotic-Antimycotic, can I make a mix of all these media supplements prior usage and...

10 October 2013 3,393 3 View

How to avoid cell aggregation/cell clumps formation after red blood cell lysis of mouse splenocytes?

Splenocytes were harvested from B6, 1X RBC lysis buffer (eBioscience) was used according to the manufacturer‘s protocol, cell aggregation was easily seen after RBC lysis but their technique...

10 October 2013 320 9 View

Any recommendation for murine BM-DCs culture and culture protocol for mix lymphocyte reaction?

I'm currently using RPMI 1640 (plus 10% FBS, 55 μM 2-mercaptoethanol, 1mM sodium pyruvate,1mM HEPES, 1mM NEAA,1% antibiotic-antimyetic ) for BM-DCs and MLR. Any suggestions to improve this...

10 October 2013 1,951 2 View