Are Agilent PL aquagel-OH MIXED-H columns (Agilent, LC1260, USA) can be used for Gel-permeation Chromatography protein analysis?
GPC is HPLC. You may use any HPLC system (e.g. Agilent 1260) with an SEC/GPC column (or columns) to attempt to resolve proteins apart. To do so, you must identify the molecular weight range (and solvent that the sample will safely dissolve into. This will allow you to narrow down the column choices) that you will be working within and then select the correct HPLC column which can be used within the same range you desire. In some cases this involves more than one column in-series, but with others, one column is able to cover the range needed.
*Note: The PL aquagel-OH Mixed Column's Mw range (for PEGs) is stated to be: 6,000 to 10,000,000. Is this the range you want? For the highest resolution, please do not use a mixed-bed column. Ideally, select one or more columns that bracket the Mw range you need. (This may be one column or it could be several in-series).
There are many GPC columns to choose from including the Polymer Labs column you referenced. Contact the many column vendors to find out which column(s) they recommend for your specific protein.
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies