I need to induce inflammation in zebrafish with carrageenan. Research papers suggesting for 1.5% of carrageenan, but I found it ineffective to induce any inflammation in them. Please suggest me an appropriate dosage.
I am looking for a cell line (preferably left ventricular cell line) to study cardimyocyte proliferation in response to different culture conditions and drugs. What will be a good cell line to use...
04 February 2018 6,640 2 View
I am trying to understand Formation of Mercaptans in Delayed Coker unit Coke Drum during entire coking cycle. Anyone has any idea how this chemistry works?
20 September 2017 6,372 0 View
Cell lysis by alternate freezing and thawing--Deproteinization with 10%, 5--sulfosalicyclic acid, centrifugation-supernatant (50 micrlitre) + 1ml Phos.buffer + NADPH (50 microlitre, 4mg/ml) +...
05 October 2016 5,743 9 View
I have a protocol that follows DTNB method for tissue and blood samples. I want to adopt this method for cell culture in combination with TCA treatment of cell pellets. Will this work?
04 October 2016 4,686 2 View
When same blood sample was taken in tri sodium citrate (1 part buffer and 9 parts blood) and EDTA (powder as such) separately, coagulation was seen in citrate buffer, but not in EDTA. What might...
25 April 2016 7,293 10 View
I want to estimate nitric oxide released by the endothelium of mouse aorta. I plan on explanting the aorta, cutting 5mm segments and maintaining them in Krebs ringers for a short while for the...
07 July 2014 944 3 View
I want to measure nitric oxide released by huvec cells following treatment with chemicals. Can I use the Griess test? I read on RG that Griess test is not the best for human cells. In that case...
07 July 2014 9,193 7 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
zebrafish and mouse
19 July 2024 9,158 1 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...
25 June 2024 4,602 0 View
Is there any equipment that replaces GPC/SEC in carrageenan characterization?
22 June 2024 2,361 3 View
Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?
13 June 2024 389 0 View
I want to teach a class using zebrafish embryos. It's in the afternoon, so I want to get my hands on some fake, reusable embryos that students can use for practice. Does anyone know of any?
10 June 2024 3,956 1 View
I did comet assay with goldfish in my master's degree but now I am experiencing such a problem. has anyone encountered such a problem before? the only difference from my other experiments is that...
09 May 2024 6,902 3 View
Hi all, suggestion for antibodies labelling proliferation that work well on zebrafish tissue? I ve used pH3 but I want something labelling more than just one phase (or labelling longer phases such...
25 April 2024 7,182 3 View
Hello everyone, I would like to know whether we can use the vibratome to section zebrafish larvae (8-9 days post fertilization) for histology. We have a Leica VT1200S vibratome in our lab....
15 April 2024 1,994 0 View