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Questions related from Niranjana Kamalapriya
Cell lysis by alternate freezing and thawing--Deproteinization with 10%, 5--sulfosalicyclic acid, centrifugation-supernatant (50 micrlitre) + 1ml Phos.buffer + NADPH (50 microlitre, 4mg/ml) +...
06 October 2016 5,755 9 View
I have a protocol that follows DTNB method for tissue and blood samples. I want to adopt this method for cell culture in combination with TCA treatment of cell pellets. Will this work?
05 October 2016 4,697 2 View
I need to induce inflammation in zebrafish with carrageenan. Research papers suggesting for 1.5% of carrageenan, but I found it ineffective to induce any inflammation in them. Please suggest me an...
28 April 2016 8,258 0 View
When same blood sample was taken in tri sodium citrate (1 part buffer and 9 parts blood) and EDTA (powder as such) separately, coagulation was seen in citrate buffer, but not in EDTA. What might...
26 April 2016 7,306 10 View