Aphids reproductive failure, what is going on?

31 August 2021 0 922 Report

Does anyone knows what is going on with my colony of Acyrthosiphon pisum? For somehow all birth is disrupted, the nymphs aren´t able to get out but still grows inside until burst the adult´s belly.

Badges
Science topic

Similar topics
Filing

More André Sarria's questions See All

How can upload an article research gate ?

I want to upload my articles drafts

29 June 2024 2,016 3 View

How can a single photon propagate in a straight-line in a dielectric medium?

Given the well-documented quantum Rayleigh scattering of single photons in a dielectric medium [1] A. P. Vinogradov, V. Y. Shishkov, I. V. Doronin, E. S. Andrianov, A. A. Pukhov, and A. A....

17 June 2024 7,692 9 View

Which is the best model for hypoxia in cell culture ( MSC or fibroblasts)?

I am tring to culture cells in hypoxia using Cobalt chloride model , but I want to compare with antoher method. Our research is about circRNA expression - qPCR and nanopore oxford sequencing.

05 June 2024 2,246 1 View

Why do you name scientists just A. Silva, as there are multiple scientists with the name Silva whose name starts with an A. ?

You sended me a couple of guestions "Did A. Silva author this work". I Looked at the article and found that just the University of Lisbon has over 10 scientist with that name.

13 May 2024 3,524 0 View

ARMAX model to Transfer Function?

Hi i have estimated an armax model using python sippy library. The estimation gives me two transfer functions H and G. How can I combine them into a single one to predict model output for new...

20 January 2024 1,221 2 View

Parasite Eggs Identification, can anyone identify this eggs?

Hello, I'm working on my MSc project related to Parasitology of Marine mammals, particularly cetaceans. I've been identifying some eggs and adult specimens by morphological methodologies, however,...

23 December 2023 2,042 1 View

Foundation models as knowledge-based systems?

Hello, during the recent emergence of LLMs and foundation models I restarted thinking about the very early machine learning attempts to incorporate human expertise and knowledge into expert...

22 November 2023 5,625 0 View

Why won’t PEG stay neutralized?

Upon receiving PEG we titrate to find its base value so it can be neutralized with HCl for certain processes. It’s typically basic on the micromolar level (I think from residual KOH or another...

10 November 2023 6,960 1 View

How to clean Titanium Foil as current collector for batteries?

Hi anyone, I am starting using Ti foil for zinc-ion battery current collector, but i want to know how to clean the Ti Foil. Thank you

25 October 2023 3,649 4 View

O que você acha da Ilha de Lixo Plástico que boia pelo Pacífico, matando animais marinhos que se alimentam deste lixo?

Nanotoxidade Ilha de Lixo no Oceano Pacífico Mortalidade de animais marinhos

22 October 2023 2,685 19 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

Why is the Protocol for Preventing Postpartum Hemorrhage (https://pubmed.ncbi.nlm.nih.gov/36113283/ ) not being widely adopted?

If the placenta is delivered in squatting position within 3 minutes of the birth of the baby, postpartum hemorrhage is completely eliminated. It works every time. It is quite likely that if a...

05 August 2024 8,895 0 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How do I compute SEM for percentages using SPSS?

I'm evaluating the reproductive performance of quails. I have the fertility, and hatchability percentages for six different sire groups and would like to determine the significant difference...

14 July 2024 2,203 5 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

12 July 2024 7,027 0 View

I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

11 July 2024 6,775 3 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

Can someone provide any tips for cell quantification on Fiji, ImageJ?

Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...

05 July 2024 4,197 1 View