Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

I induced diabetes with STZ (40 mg/kg) after 4 weeks of high fat diet in Wistar rats. The rats are showing inconsistent blood glucose level. Why?

I induced diabetes with STZ (40 mg/kg) after 4 weeks of high fat diet in Wistar rats. After 72 hours, i checked the FBG of all the rats and majority of the rats had FBG above 350 mg/dl. After a...

24 September 2020 5,975 24 View

Global warming challenges, what's the way to go?

New discussion While some still consider climate change majorly caused by GHGs as a hoax, it is impossible to rule out a striking correlation between the increasing concentration of atmospheric...

28 October 2019 9,301 24 View

Can Microplastics act as vector of chemical transport into human system on the model of stomach oil extraction ?

Chemical transport from micro-plastics to animal tissues has been reported in birds.

01 August 2019 9,216 1 View

I need more article on the assessment of nutritional status in school age children?

01 July 2019 6,210 2 View

How do You determine the ranges of value for your parameters, time and temperature using soxhlet extraction(N-hexane And Ethanol) for lemon grassoil?

I'm trying to conduct an experiment on the optimization of extraction of lemongrass oil using soxhlet extraction. My Optimum parameters are temperature, time and lemongrass to solvent ratio. I'm...

08 April 2019 9,256 1 View

Is it possible to connect dc motor directly to dc bus?

I want to know if is possible to connect dc motor directly to dc bus. I don't know if is possible to eliminate the converter and make use of DC Motor directly.

26 March 2019 3,602 5 View

Can I pool sera of chickens within a replicte together for LH FSH ELISA analyses in order to reduce cost of analyses?

I need to run ELISA analyses for reproductive hormones (LH, FSH) in chickens. The samples are however too many. Can I pool sera of birds within a replicate/treatment together for the analyses in...

19 August 2018 9,819 2 View

How do I plot my data using the Sigmoidal Langmuir equation?

I've included an image with showing the equation. I need to know what parameters to plot on the x and y axes. Any help will be highly appreciated.

15 January 2018 290 3 View

How do I go about Maxi Prep Preparation?

12 June 2017 3,060 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Plant extracts on expression of virulence related genes?

Good day. I would like to extract total RNA using a kit from MRSA and candida for gene expression. i tried sub-culturing standardized inoculum (50ul) with 500ul of my plant extract sub-MIC and...

23 December 2020 5,076 3 View

Biotech final year project for a bioinformatics beginner?

So my last year project is Drug Efflux Pumps and Persistence in Methicillin Resistant Staphylococcus aureus and we gonna focus on persister cells to study the path way of antimicrobial...

09 December 2020 2,574 2 View

Prediction of novel precursor miRNAs in viral genomes?

Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...

06 December 2020 1,704 3 View

Who can i take a smear from inhibition zone?

I used alcoholic extract of peganum harmala seeds as inhibitor to staphylococcus enterotoxin type A gene (SEA). By using diffusion method I need to know how can i take a smear from zone of...

29 November 2020 3,165 3 View

If a person acquired MRSA deeper into the skin, is it possible to not have signs and symptoms? What will happen if it will be left untreated?

Methicillin-resistant Staphylococcus aureus (MRSA) is a type of staph bacteria that has developed an immunity to antibiotics commonly used to treat staph infections. MRSA is usually not detected...

20 October 2020 8,473 8 View

MsaClustalW() Take ages. What to do ?

Hello, I'm new to the bioinformatics world and would like a feedback on which MSA tool to use. I want to find a consensus sequence in about 18000 sequences (from marine biology samples) from...

19 October 2020 2,607 2 View

Which of the ff. solvent is more effective to use as a bactericidal agent in antimicrobial testing of staphylococcus species, ethanol or methanol?

The antimicrobial activity testing of staphylococcus species.

02 October 2020 6,996 7 View

Resistance mechanisms- MRSA?

I need to describe resistance mechanism of MRSA and would appriciate if any could recommend an article (relatively new review/summary)?

02 March 2020 1,308 2 View