Apart from DNAmethylation and homologous recombination frequency, is another easier method of detecting transgenerational adaptation in sress plants?

22 February 2018 0 2K Report

Transgenerational adaptation to heavy metal stress have been reported, which could be in the form of DNA-methylation or Somatic recombination frequency. How else could transgenerational adaptation be detected in plants?

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Science method

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Given the current level of natural phenomena cause by the climate change and environmental pollution, will the AI find the technological solutions?

AI-driven technologies offer previously unheard-of capabilities to process enormous volumes of data, extract insightful knowledge and improve predictive models, according to the UN’s World...

31 July 2024 9,733 3 View

Is it okay to grow bacteria in 30 for cloning? What is the lowest mass of Vecor I can take for cloning? what should be the optimum insert ratio?

I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...

26 June 2024 3,735 3 View

Why is the blue and white spot screen negative for all white spots?

The protein was expressed using the insect cell system, and the recombinant plasmid was successfully constructed as verified by sequencing, and the recombinant plasmid was transformed into DH10...

13 June 2024 2,590 4 View

What are the primary mechanisms through which green bonds contribute to climate change mitigation and adaptation?

climate

11 June 2024 3,719 1 View

Is a 5` phosphate needed for homologous recombination?

Is a 5` phosphate needed for homologous recombination? I am a bit confused whether phosphorylated DNA is needed to achieve efficient homologous recombination in e coli. I believe there are methods...

06 June 2024 7,065 1 View

How can the Global South effectively participate in climate change mitigation and adaptation in the Arctic and Antarctica regions?

The Global North primarily leads in climate financing, technological capacity and related matters. The Global South is mainly perceived to be a "victim." How can this mentality be re-engineered?

04 June 2024 1,854 2 View

How parasites have evolved with adaptation to coexist with their host in an ecosystem and ecological significance of parasites?

31 May 2024 9,855 5 View

How can climate change adaptation strategies be integrated into agricultural policies & practices at local, national, & global levels & global levels?

How can climate change adaptation strategies be integrated into agricultural policies and practices at the local, national, and global levels?

21 May 2024 2,817 8 View