Is there any miRNA fluorescent labeling kit (or methods) that does not affect (add) the length of the miRNA?

I want to label my target miRNA and see the miRNA trafficking. But I don't want to use any plasmid or radioactive label. I need the fluorescent label that will not affect the length of my...

06 December 2020 8,471 3 View

Can too much of Tris-Cl buffer inhibit PCR?

I accidentally resuspended my stock primers (100 uM) in tris-Cl (pH 8.0) 1 M instead of 10 mM. I do PCR with 20 uM dilution from the stock. Will the high concentration of Tris-Cl inhibit PCR? I...

12 July 2020 2,971 10 View

Does hot KOH react with zirconia?

Hot KOH destroy many materials like alumina or glass.. So, can I use zirconia(ZrO2) boat for KOH activation of carbon nanoparticle experiments?

09 April 2020 800 1 View

Paired t-test or Independent samples t-test?

Hi, I am working on the home advantage. And examining the difference of a player's performance between playing at home and away. However, the sample size is different because the player played...

24 February 2020 7,053 3 View

Is it possible to wash PPFE slides that have been put through fluorescent reaction and stain them again?

I have some 3 year old PPFE slides of human brain that have been reacted with primary antibodies then reacted with Alexa Fluor secondary antibodies. The DAPI on the slides is still visible but the...

02 May 2019 7,390 2 View

What type of statistical tests on SPSS should I use for my study?

Hi I am carrying out a study investigating the association between age and password strategies among online users. I have collected my data, and inputted it on SPSS, however, I am not sure which...

10 December 2018 1,002 7 View

Can docking be used to predict a peptide's target receptor?

Let's say I have an array of receptors. Given a peptide, can protein-peptide docking be used in predicting the peptide's target among the receptors? If so, how? If not, why? Can you recommend me...

24 July 2018 3,569 4 View

Fc block not working for Flow Cytometry?

Good day all! I would greatly appreciate help with the following issue: Our lab is trying to characterize mouse lymphocytes for Tbet, EOMES, and PD-1. When I ran the samples the first time, the...

28 March 2018 1,225 3 View

In general speaking, how long can I keep antibody-labeled sections in wash buffer after the staining of secondary fluorescent antibody?

If the mounting is delayed for a day or two, would the specific binding being greatly reduced?

05 December 2017 4,103 3 View

Call for Papers: Australian Gender Economics Workshop (AGEW), Feb 2018, Perth Australia ?

15 October 2017 9,870 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View