Anyone who performs PCR for Diphyllobothrium?

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How can I publish my final master project as an article for a scientif journal?

I am interested in publishing an updated version of my final master´s project in Spain and i am not sure which steps to take. I am open to suggestions.

16 July 2024 7,431 1 View

¿Why increasing the effective surface area decreases the current density in microelectrodes?

To characterize the electrocatalytic activity of the microelectrodes, the effective surface area was determined using the equation proposed for cylindrical microelectrodes by (Szabo, 1987). But, I...

14 January 2024 8,116 3 View

Gaussian NMR calculation error: Bad dimensions in Pcck?

Hello gaussian users I'm trying to calculate a NMR spectrum of a macrocyclic compound, I was able to optimize the molecule but not able to get the spin-spin couplings. The program shows this...

07 January 2024 3,223 0 View

Relationship between the Austrian theory of economic cycles and the bullwhip effect?

Is there any relationship between the Austrian theory of business cycles and the bullwhip effect in the supply chain?

05 January 2024 1,754 1 View

Statistically what has been the role of upper management in staff turnover and burnout in social services field, pre-pandemic, post-pandemic?

I am researching rampant middle staff resignation in NYC throughout homeless shelter and supportive housing agencies after the COVID-19 pandemic and the resulting consequences for the clients...

14 October 2023 1,885 1 View

¿Como se fundamenta la fitodepuración del nitrógeno mediante el uso de la lenteja de agua?

Referido al contexto de tratamiento de aguas residuales, quiero saber el comportamiento de la macrófita y toda la teoría que engloba para eliminar del agua compuestos nitrogenados.

30 July 2023 8,439 1 View

I would like to correct the title of a chapter that I've recently published. How can I do it?

Perhaps the title was too long: "A Gulbenkian e a acção de Madalena Perdigão. To cut the name Madalena (Perdigão) is not an option!

04 July 2023 2,342 2 View

One title cut?

My chapter A Gulbenkian e a acção de Madalena Perdigão is seen in your site without the full name Madalena Perdigão. I changed it to private because of this issue. Could you please send me an...

29 June 2023 2,781 0 View

Which duplex is more stable, RNA:RNA, RNA:DNA or DNA:DNA?

I have been looking for some papers talking about duplex formation but it is still unclear for me which is the strength of formation of these duplexes or if it is sequence-dependent. If you could...

05 June 2023 6,182 3 View

¿Qué es desarrollo regenerativo?

En el marco de la ecología y protección del medio ambiente

19 April 2023 9,185 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View