Anyone who performs PCR for Diphyllobothrium?

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How Can I solve this mistake in autodock?

ERROR ********************************************* Traceback (most recent call last): File "C:\Program Files (x86)\MGLTools-1.5.6\lib\site-packages\ViewerFramework\VF.py", line 898, in tryto...

15 November 2020 1,479 2 View

How could I use Solvate gromacs command for solvate on Z axis of a monoclinic box, whitout living water molecules outside?

Currently I use Gromacs to perform molecular dynamics of tobermorite 11A, but it turns out that this structure has a monoclinic box, I need to solvate my structure only above in the z axis, but I...

26 March 2020 1,208 3 View

Is there a principal responsible for water management?

The water crisis is a situation that affects us all, however: Is there a principal responsible for water management?

16 January 2020 8,671 6 View

IHC perfused brain tissue disintegrating following TBS wash on Super Frost Slide?

Hi all, I am using IHC to look at various neuronal markers in zebra finch brain tissue. Finch brains are perfused with PBS and then 4% PFA and placed in a sucrose solution overnight. I then...

01 January 2020 8,400 3 View

What is the expiration of the RPMI 1640 medium once the FBS and antibiotics has been added?

I had a medium stored and sealed for 2 months that presents an intense pink color, I put it in the CO2 incubator but it does not recover the light pink color. I will make a new medium but, has...

10 November 2019 1,632 2 View

What do you think is the most commonly used method for systematic literature review?

In my case I consider that it is the PRISMA, it has many advantages among them it allows to improve the report of the systematic reviews and of the meta-analyses. They adapt to tools such as StArt...

09 October 2019 2,769 4 View

Any source for real time control in PYNQ boards?

Real time control in parallel robots is a requisite. Python has popularity for scientific computation thanks to Scipy and Numpy libraries. Hardware implementation of control routines reduce...

21 July 2019 3,783 1 View

How to calculate the empirical power to compare the performance of two FANOVA tests in functional data?

Hello, do any of you know how to calculate the empirical power of a FANOVA test in functional data analysis? or if anyone knows a script to calculate it.

16 January 2019 10,042 2 View

Does anyone could point me in the right direction in order to organise my database using Excel?

I am conducting a Meta-analysis about forest permanent sample plots on different vegetation types in order to analyze different plot-sizes as well as the minimum diameter-size recorded on the...

31 December 2018 8,741 2 View

Can someone recomend me articles or books to identify Pacific marine sponges?

I'm helping to identify marine sponge specimens from the Pacific of Costa Rica.

31 December 2018 4,228 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View