Available products carry mutations in endonuclease genes cause they are used for cloning applications. Please advise. Thank you!
There are many commercial sources of E. coli strains expressing the EcoRI enzyme. You can try contacting companies that provide DNA cloning services or online biological reagent suppliers like Addgene, Thermo Fisher Scientific, or New England Biolabs (NEB). You can also search for scientific collaborators or research groups who have worked with this enzyme in the past and request the bacterial strain from them.
Additionally, you may want to consider cloning the EcoRI gene into your own favorite E. coli expression systems, such as pET or pGEX vectors. This approach will give you greater control over the expression levels of the enzyme and allow you to tailor the purification protocol to your specific needs.
Keep in mind that working with live bacteria and recombinant enzymes carries some risks and it is important to follow the appropriate safety guidelines for handling these materials in a laboratory setting.
yes you can purchase from Addgene directly.
It is not clear what you mean by "Available products carry mutations in endonuclease genes"
Please note that the EcoRI gene is not an endogenous E. coli gene but rather is from an E. coli plasmid found in some strains.
You can purchase EcoR1 enzyme from various scientific supply companies such as Promega, New England Biolabs, and Sigma-Aldrich. You can also find bacteria expressing EcoR1 from ATCC and various other suppliers.
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