01 January 1970 1 6K Report

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More Franklin Escobar-Córdoba's questions See All
Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

Is there any method to prevent extracellular vesicles from aggregating?

Hello colleagues, I am trying to isolate extracellular vesicles, which tend to aggregate. Is there any method to prevent them from aggregating?

04 October 2023 1,024 2 View

Do you have issues running the light curve with your phyto US unit for Phyto PAM?

Hello you all, hope you are fine Well, I'm working right now meassuring different parameters of photosyntesis and I was interested in use a Phyto US unit for a PhytoPAM, the unit seems right and...

14 September 2023 788 0 View

How Air Liquid Interface organoid cultures preserve stromal components?

Hello! My name is Laura. I have been working on the generation of colon organoids from patient biopsies for the last two years. I follow standard protocols, first I isolate intestinal crypts and...

21 January 2023 5,774 0 View

Has paper rejection rate increased in COVID times?

Has anyone else also felt that editors are rejecting more papers during this pandemic times?

30 July 2020 382 32 View

Best housekeeping gene for cells cultured qPCR?

Hello all, What is the best housekeeping gene to use on real-time PCR using cells cultured and subjected to different doses of ionizing radiation? Thank you. Elisa

14 January 2020 6,314 4 View

How much time can be detectable the domoic acid in a water sample?

Greetings! I am working with Pseudo-nitzschia sp, and I want to check some DA concentrations dissolved in samples or sea water, but I don't know how much time can the domoic acid be detectable...

08 April 2019 6,485 4 View

Does anybody know any academic established in a Turkish university to start a collaboration on insect pheromone characterization?

There is a new research collaborative program between Mexican and Turkish governments and I would like to apply. My interest is related to characterize triatomine pheromones to control chagas...

02 August 2015 2,775 3 View

Do you know cases in your country dealing with the (copyright) "three-step test"?

Although created more than four decades ago, the so-called “three-step test" has grown in importance over the last years since it began to be used in response to the abuse of the copyright...

16 December 2013 6,642 5 View

Does anyone know studies of how changes in male tactics impact female fitness?

With some colleagues from Simon Fraser University, we are developing a mathematical model to understand the evolution of male mating tactics in the framework of female fitness. It would be nice to...

05 February 2013 1,203 4 View

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Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

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Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

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Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

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How to make Boehm titration ?

Hello Everyone, While doing Boehm titration, We use nahco3 in order to determine carboxylic acids. Then the mixture was agitated. After a while, the solution was filtred and HCl was added. After a...

29 July 2024 4,048 4 View

Medical question: Is OSA the cause of Afib and HTN?

Does Obstructive Sleep Apnea either cause or exacerbate Atrial fibrillation? Hypertension? We know they are comorbid factors in 50-70% of all OSA patients, and with OSA being so hard to detect...

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Can you recommend provider Intermediate laboratory test for hydrocarbon, polyethylene & polypropylene?

Accredited laboratory of JV "Uz-Kor Gas Chemical" LLC (Uzbekistan) is looking for providers to conduct interlaboratory comparison tests on gas chromatographic analysis of hydrocarbons (component...

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How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

What analysis to use for an dependent variable with repeated measures and a independent variable only measured once?

Hi all, I am trying to use mixed effect model to analyze my data, which including a baseline measurement for my exposure (A), and repeated measurements for the outcome (B). I do have some...

17 July 2024 8,682 3 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

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How to bring baseline to zero for an absorbance data for chromatogram?

I forgot to autozero during the run (Size exclusion chromatography.) and later i realised i forgot to do that and the baseline was not zero but below zero (and in some cases it above zero). I...

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