How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Calculate the Strain at a Node in the Turbine Blade FE Model?

Hello Team, I would like to calculate the strain at a node #5 in a direction (l1,m1,n1) in the turbine blade FE model as shown. This node is connected with four elelemnts (#1,#2,#3,#4) and I have...

11 January 2021 7,702 3 View

One invention granted for the US and European patent will be counted as one patent or 2 patents for CV writing?

One invention granted for the US and European patent will be counted as one patent or 2 patents for CV writing?

05 January 2021 8,135 4 View

Buckling of Smart composite plates using FEM ( MATLAB code)?

Can anyone help me with the FEM coding for Buckling of composite plates using the FSDT or HSDT - Source or the helping material (textbook, research paper etc) - Geometric stiffness matrix...

03 January 2021 3,059 3 View

How to optimize a structure for excited state when there is a bond breaking during optimization in gaussian?

In Gausssian, I have tried to reoptimize that structure by freezing the bond which is breaking in excited state during optimization. Before freezing the bond i have already tried scf = xqc and scf...

20 December 2020 8,444 3 View

Could not get reduction peak for Mxene Counter electrode in Iodide electrolyte, why?

I have deposited MXene Ti2C2Tx mixed with tritonX binder and heated at 250 C for half an hour in ambient air conditions on FTO glass substrate, the deposition technique followed was doctor...

28 November 2020 2,330 6 View

What are the best binder which can be used for deposition of MXene on FTO?

I would like to coat MXene (Ti3C2Tx) on FTO glass substrate using doctor blade deposition technique, for which i think a binder is essential for the adhesion of MXene onto the FTO. The post...

28 November 2020 9,360 3 View

How to build a polymer pdb file from a monomer pdb file, for using it is an initial configuration in Gromacs?

I am doing my Ph.D. in Materials Science and am completely new to Molecular Dynamics Simulation and Linux. I am using Manjaro Linux 20.1 and have Gromacs 2020.4, VMD 1.9.3, packmol 20.0.10,...

28 October 2020 1,051 3 View

Kindly suggest the best organic solvents used for dispersibility of graphite powder?

Kindly suggest me some good papers regarding a comparative study on various organic solvents used as dispersion medium for graphite powder. The application is to make a graphite paste and used the...

24 October 2020 1,586 4 View

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02 March 2021 3,060 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View