Anyone involved in Lifestyle Medicine ?

More Daniela Assimiti's questions See All

Trypan Blue overestimates the viability of cryopreserved PBMC?

Hello! Have you ever observed that when measuring the viability of freshly thawed PBMC with trypan blue, the value is higher than with Annexin/PI staining or some other staining that takes into...

10 July 2024 640 1 View

How to do a systematic review?

Hello, I am working on a systematic review. I have my topic, and I am searching Scopus and Web of Science, but I want to search Google Scholar to compare the results. Does anyone have any advice...

10 June 2024 9,602 0 View

Maximum passage for Ewing sarcoma cell lines?

I was hoping anyone could provide any insight into using ATCC's "RD-ES" and "A-673" cell lines. How many passages do you do before you notice any senescence or genetic abnormalities

20 May 2024 596 4 View

Research about impact of brave and persistence?

I want to read about studies or experiments who proved that being brave and persistent are suceeding factors. Are there any new aspects or findings e.g. from neuroscience?

12 May 2024 4,614 3 View

Experience on subculturing and feeding RD-ES cell line?

Hi - I am looking for advice on protocol for subculturing and feeding RD-ES line. THey seem to grow semi-adhered and not sure if I should treat them as cells in suspension or adhered. Thank you!

09 May 2024 8,224 1 View

Hello everyone! Would you please help me identifying this marine polychaete ?

It was extracted from a sediment sample in the subtidal zone

19 April 2024 6,725 4 View

How to perform the embedding and cutting of 0,4 um transwell Pet inserts to visualize polarized cell layer for histology?

i seeded CACO2 on 0,4 um PET transwell insert, i want to see my cell layer in transversal view in order to monitor the polarization and formation of brush border. To do this, i fixed with 4% PFA...

16 April 2024 6,517 1 View

How can I induce Netosis cell death in HL60 differentiated with DMSO?

Months ago i was able to induce NETosis in Hl60 differentiated cells using PMA while now with a different PMA solution I can't anymore. I followed the condition reported in literature of 100nM PMA...

18 March 2024 2,925 0 View

How can I extract RNA from a vine leaf?

Greetings! I started my thesis and I need to research on the methods to extract RNA from vine leaves.

05 March 2024 1,162 3 View

Could anybody help me with my human macrophages primary culture?

I am differentiating monocytes into macrophages from PBMCs, and every time I have encountered these bacillus-shaped elements when I looked them in the microscope at 40x. They are not typical...

28 February 2024 2,710 2 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Have you heard about Technological Surveillance on Oceanographic Research Vessels?

It is important to highlight that technological surveillance on oceanographic vessels is a collaborative effort in which different specialists work together to effectively leverage technology in...

31 July 2024 1,633 0 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

How can I find writers for drafting, submitting, and publishing papers?

Looking for paper collaboration writers： Collaboration Model 1: 1.1 Based on the chosen topic, complete the paper writing, select an SCI or SSCI Q1 journal, submit using my QRCID, and complete...

27 July 2024 8,965 1 View

How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

Who wants opportunities for scientific cooperation?

Dear Colleagues, I hope this message finds you well. My name is Noor Al-Huda K. Hussein,and I am a researcher specializing in deep learning applications in genetic data analysis. I am currently...

18 July 2024 5,562 0 View

Who wants opportunities for scientific cooperation?

Dear Colleagues, I hope this message finds you well. My name is Noor Al-Huda K. Hussein, and I am a researcher specializing in deep learning applications in genetic data analysis. I am currently...

16 July 2024 3,981 6 View

Oncologist at Visakhapatnam interested to collaborate?

Calling all medical professionals and researchers! I'm seeking recommendations for an oncologist in Visakhapatnam who can collaborate on my PhD thesis. My research focuses on stage 1 and 2 gastric...

14 July 2024 936 0 View

Interested in Research Collaboration?

anyone looking for research collaboration on the anaerobic digestion slurry and digestate can contact me for further details. As my whatsapp number is +91 8317585217

11 July 2024 6,753 3 View