Whether the X-ray source and electron source must be coherent for XRD and electron diffraction?

Whether the X-ray source and electron source must be coherent for XRD and electron diffraction? For X-rays emitted from X-ray tubes and electrons from electron guns, the photon and electron...

01 July 2020 540 9 View

How to get the eqution of the covariance matrix Rk based on residuals?

How to get the equation Qvxk=Rk-HkQxkHkT by applying the error propagation law to vzk=(E-HkGk)dk?

10 December 2019 8,315 1 View

How can I search a substructure in a big structure?

Hi, I have a cubic MD simulation box containing 56 molecules, the positions and orientations of these molecules will change during the simulations, and I have another crystal cell containing...

31 July 2019 3,021 1 View

Can you recommend me some chapters and articles about III-V solar cell manufacturing?

I want to learn the fabrication of III-V multijunction solar cell details.

03 December 2018 3,490 3 View

Why sometimes in EN measurement [ZRA mode], only current or potential signal shows transients, not simultaneously in both current and potential data?

Hi, I recently got an EN record as shown in the attached image. The carbon steel specimens were uniformly corroded in general, but there were a few areas that were corroded more seriously - the...

28 February 2018 3,707 2 View

A shift in RT in my HPLC, please help me?

I used C18 semi-prep column to purify natural products. I've found the great gradient for my compound purification and ran 2 run for 50um of my sample and I have wash procedure after each sample...

06 February 2018 2,520 2 View

How to distinguish the direction of subduction by foliation occurrence of the accretionary wedge?

01 July 2017 4,283 2 View

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26 May 2017 7,984 2 View

What effects temper martensite embrittlement have on fatigue of steel ?

what effects temper martensite embrittlement have on fatigue crack nucleation and propagation?

10 October 2016 1,576 25 View

How can I measure cholesterol and triglycerides level in mouse liver?

we did plasma measurement of cho and tri. in our mouse samples, now we would like to measure direct level in the fresh tissues, does anyone know where we could do this around montreal area, or any...

12 July 2016 6,187 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Compact antenna for the frequency range 1 MHz-5 MHz?

Hello everybody! I have a problem with developing a compact antenna for the 1MHz-5MHz frequency range. Tell me what type of antenna can be used and what books and articles to read to perform this...

27 February 2021 7,710 4 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View