anyone inject cytokines into mouse directly?

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Could Isight run ABAQUS files that use subroutines?

During the process of using Isight optimization, it ends by modifying each of the seven parameters that need to be optimized. And the target parameters have never changed, I suspect it's a problem...

18 December 2023 6,079 0 View

How to change the representative colours Traction Force Microscopy image?

Dear all researchers, I have gone through many articles about Traction Force Microscopy, however, I wonder if anyone could provide me with some advice regarding how to change the representative...

05 December 2023 1,067 2 View

How to distinguish the martensite/bainite K-S relationship variant pair, V1/V2 and V1/V3?

Hello fellows, I'm a Ph.D student studying martensite/bainite crystallography, there has been a question troubling me for some time. We know that for FCC/BCC K-S relationship, variant1 and...

03 July 2023 8,792 2 View

How to define user model by user procedure in Aspen Adsorption?

There is an unique existence that the user procedure method is the only way to apply user's model when estimating molecular diffusivity coefficient. So I compiled the fortran code with the...

18 June 2023 3,614 0 View

Is there a non-invasive way to monitor tumor bone metastasis?

Dear peers, I have a technical question about monitoring tumor bone metastasis. We want to establish a tumor model of bone metastasis and monitor the metastatic procedure. I am aware that IVIS...

16 May 2023 1,693 0 View

Which method should I use to build a phylogenetic tree of the Arabidopsis protein family? Maximum likelihood, neighbor-joining, or else?

And should I also include an out-group? This is my first time doing it, and I need some guidance. Thank you!

16 May 2023 3,649 1 View

Any suggestions for non-invasive small animal imaging equipment?

Dear all, Is there any equipment that can track the drug distribution in the mouse? We want to see if the drug or small molecules labeled with a fluorescent dye are enriched in the targeted...

10 May 2023 8,074 0 View

What is the mechanical mechanism of the air waves caused by high-speed landslides?

Landslide is a huge disaster.

05 May 2023 5,656 1 View

What is the triggering mechanism of high-speed remote landslides?

Landslides are a huge disaster.

09 April 2023 9,348 5 View

What is the dynamic mechanism of earthquake induced throwing landslides?

Landslide is a huge disaster.

07 April 2023 5,458 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View