Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

CUDA Out Of Memory (OOM) error while using GPU?

I'm training a neural network (E3D-LSTM) for spatio temporal video prediction. It's quite a big network that has millions of parameters. The model is training perfectly when using my 12 CPU cores,...

25 December 2020 1,229 3 View

How can I model Solar PV, Battery from Electric vehicles, and grid in Matlab for cost minimization?

How can I model a system in Matlab consisting of Solar PV, Several Batteries from Electrical vehicles, and a grid altogether so that the load demand is fulfilled in such a way that the cost of...

20 November 2020 8,580 4 View

How can I model three types of power sources which are fossil power from the grid, green power from the solar panel, and green power from the PHEV?

How can I model three types of power sources which are fossil power from the grid, green power from the solar panel, and green power from the PHEV from cars of 100 employees (who come in the...

12 October 2020 4,720 3 View

How many internet Switches are there between one country to another country?

How many internet Switches are there between one country to another country?.

11 October 2020 1,034 3 View

When can we use Fisher's Least Significant Difference (LSD) test?

Hi, Following a two-way analysis of variance (ANOVA), when can we use Fisher's Least Significant Difference (LSD) test? Is it reliable or explainable showing data? I have some mouse behavior data...

28 September 2020 9,374 5 View

Which area i should focused in FSW of Al-Steel with interlayer?

Can anyone give me suggestion about topic to carry my research? As i see there is number of publication in this area.so i am not getting that which objective i should keep for my research.

04 September 2020 2,286 2 View

Comsol electromagnetic simulation. How to give a mesh for certain micrometer when my tool is in millimeter. The current density is so small. Pls help?

Hi, I am trying to perform mesh convergence and I found out there is no stable temperature value I obtained. So, I calculated the skin depth and it was around 0.00011mm. It is so small in...

24 August 2020 9,060 2 View

Which option (SFA-Cross Or DEA-DMU) is best to measure the individual efficiency (for a sample size of 450 farmers)?

I am working on a set of data, collected from a field survey. I am looking forward to measure the the level of technical efficiency of type of irrigation channels (Solar pump set/ Diesel pump set...

06 August 2020 2,510 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View