We want to inhibit the binding of BDNF and NT-4 to its receptor. So we are looking for some peptide inhibitor or antibody that can binds to these proteins in their binding sites. Any suggestion? Thank you.
Thank you for your answer. In this paper, as others that we found the inhibition target is TrkB. But we need to block specificly BDNF and/or NT-4.to know which is the neurotrophin responsible for intracellular signaling...
You could use an anti-BDNF neutralizing antibody, there are several commercially available options
Hi Erica, I run a neurotrophin lab as well as act as an advisor to Biosensis (biosensis.com). They have some excellent blocking antibodies to all the neurotrophins that are well characterized in terms of cross-reactivity, dose, etc. The best characterized are sheep antibodies. I suggest you contact [email protected] to discuss with Dr Lyndon Foster, or myself. Please note that I declare that I am also a shareholder in Biosensis.
Thank you for your answers. We visit our website and these antibodies are a really good opcion for us, Thank you once again.
Antibody #9 is a great BDNF neutralizing antibody developed by Yves Barde. It is very affordable and available through the Hybridoma bank at the Univ of Iowa (http://dshb.biology.uiowa.edu/brain-derived-neurotrophic-factor). They do ship internationally. Alternatively, K252a will block Trk signaling. Although it is a dirty drug, it is an easy tool to test and support your hypothesis. Also the dominant negative T1 TrkB has been use to shut down signaling if you can transfect the construct in your system. Good luck!
SATPdb: a database of structurally annotated therepeutic peptides.
http://crdd.osdd.net/raghava/satpdb/
paper link
http://nar.oxfordjournals.org/content/early/2015/11/01/nar.gkv1114.full
Number 9 does not work well in traditional westerns. Its companion, #1 works better as was demonstrated in the Kolbeck J. Neurochem., Val. 72, No. 5, 1999 paper. IP will work with #9 though if this helps.
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