Anyone have experience in/paper suggestions for detecting pathogen presence by extracting viral DNA? Trying to avoid RNA extracts + cDNA library prep?

13 July 2018 1 3K Report

I'm working with birds and I'm interested in characterizing several pathogens and their load. It's a mixture of viruses and blood parasites. I'm not collecting the blood samples/lots are already collected and I was wondering if anyone had paper suggestions or experience in extracting viral DNA for this kind of project? My samples are stored/collected in ethanol tubes and are generally just whole blood. Therefore, I'm trying to avoid RNA extractions/reverse transcription PCR. Please help! :)

Renas Isa

Dear Lauren,

it depends on type of virus you are looking for. For example, if you were looking to detect Ib or newcastle viruses there is no way to avoid RNA extraction. However, if you were interested in adinovirus, there might be a chance to depend on the DNA.

good luck with your project

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