I am intending to use cryo-preserved undifferentiated HepaRG cells for liver metabolic stability studies, but have a few questions before getting it started. Hopefully someone with experience can help me out.
1. Can those cryo-preserved undifferentiated HepaRG cells from commercial source be expanded and frozen down in multiple vials in liquid nitrogen, just like any other immortalized cell lines?
2. Can I differentiate the undifferentiated HepaRG cells and cryo-preserved themfor future use, like those differentiated HepaRG cells from commercial sources?
3. Can I use 2% DMSO to differentiate or have to use the maintenance media?
Thank you very much!
Hi Esther,
HepaRG cells are very difficult to ''control'' at the proliferation phase. in my experience they tend to start a form of differentiation after they rise a tight confluence stage. For that I would not recommend them for metabolic studies at undifferentiated stages given the difficulty of controlling the expression of metabolic enzymes at that stage.
Good luck.
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