With the recommended medium and supplements, time for subculturing, etc?
Hi, maybe you can refer to these 2 articles:
1) Eosinophil Purification from Peripheral Blood. Article Eosinophil Purification from Peripheral Blood
2) Isolation of Human Eosinophils. Chapter Isolation of Human Eosinophils
Hi,
You can isolate them from peripheral blood, but they will die after a few days in culture, even if you add IL-5 to improve their survival (these cells do not proliferate).
Do you have any idea of how many hours, do they survive in culture?
Ok, thanks for the information. By the way, do you have any information regarding the necessary conditions to grow them and media requirements? Do they need movement during their incubation?
I was used to culture them in RPMI1640 +1%antibiotic-antimycotic +1% L-glutamine (200 mM stock). Eosinophils are usually just plated in multiple well plates (not tubes) without agitation. Incubate at 37°/ 5% CO2 in a humidified incubator as usual.
I was reading my former answer: viability is approx 60% after 12h and 40% after 24h, I wrote the opposite!
- Badges
- Science topic
- Similar topics
- Social Science
- Media
- Journalism