Anybody ever used Lr47 gene protocol from WHEAT CAP? I used Lr47 protocol from WHEAT cap. And PCR time was 55 min. How was yours? Can explain PCR product concentration also? Thank you
Using ready-to-use MS medium, and put in Raypa AES-75 autoclave for 20 min at 121C according to the protocol. But it becomes yellow when it gets cold. Even tried with aluminum foil. What can be...
01 February 2016 1,933 8 View
5M of NaCl is taking too many hours to dissolve in water? What can be the reasons? Is it normally like that or what else???
31 December 2015 8,937 1 View
Before running PCR what is the ratio of Taq, dNTP, Primers, buffer, water and DNA? is there any method for calculating?
11 December 2015 3,503 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View