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Anybody ever used Lr47 gene protocol from WHEAT CAP? I used Lr47 protocol from WHEAT cap. And PCR time was 55 min. How was yours? Can explain PCR product concentration also? Thank you
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Using ready-to-use MS medium, and put in Raypa AES-75 autoclave for 20 min at 121C according to the protocol. But it becomes yellow when it gets cold. Even tried with aluminum foil. What can be...
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Before running PCR what is the ratio of Taq, dNTP, Primers, buffer, water and DNA? is there any method for calculating?
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