Is it possible to perform a Set-ESEM analysis using R?

I am thinking in applying the Exploratory Structural Equation Modelling (ESEM) technique to evaluate the factor structure of a personality inventory. I have recently read about Set-ESEM, which...

10 June 2020 228 5 View

What are the best tools to create Feedback Forms related to Excel sheets?

I want to gather feedback from the implementation of processes in order to optimize them and I was wondering what tools other than 'google forms' could allow me to implement them in a formal and...

23 December 2019 8,304 3 View

Is it possible to introduce a specific mutation (by PCR mutagenesis) into a Multibac construct?

Hello. The laboratory where I work has received a pFL Multibac construct from another lab. This construct contains apart from the vector backbone, four of the genes that we are interested....

26 September 2017 7,881 3 View

How can I obtain a SEM image in order to verify columnar growth in Sputtering?

08 September 2017 5,746 5 View

How can I obtain a SEM image in order to verify columnar growth in Sputtering?

31 August 2017 265 4 View

Any research on model calibration using a game-based interface?

11 December 2016 9,210 3 View

Any research on model calibration using a game-based interface?

05 December 2016 6,058 3 View

There is some research about using Reinforced Learning for content suggestion?

08 March 2014 8,387 1 View

There is some research about using Reinforced Learning for content suggestion?

02 March 2014 5,706 2 View

Hi, I somebody have information and protocols for doing IHC-Fr with the Ab Ki67?

Which is the better way in order to label IHC-Fr brain section of 20um on glass with Ki67ab

01 January 1970 5,043 6 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View