Hello everyone.

I'm trying to run an Inhibition Elisa of two purified allergens from D. pteronnysinus at the same time. So, for this, I coat the microplate with the two allergens and then, for the standard curve, I use them in the same proportion. The results have got too much variability between assays. Does anybody knows if it is possible to analize two allergens in the same Inhibition Elisa?

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