Dr Arwa,

Are you asking about Ethidium Bromide decontamination and disposal procedures? If this is your query, then you can refer to the following sites:

http://www.ncku.edu.tw/biochem/E-S-H/SMOU-Cleanup_of_Ethidium_Bromide.pdf

http://documents.manchester.ac.uk/display.aspx?DocID=15543

https://www.slu.edu/research/faculty-resources/research-integrity-safety/environmental-health-safety/documents/ethidium-bromide-use-and-disposal.pdf

regards,

SD

Some more links:

https://ehs.utk.edu/wp-content/uploads/2017/06/Ethidium-Bromide-Waste-Guide.pdf

https://www.clontech.com/CA/Products/Nucleic_Acid_Purification/DNA_Clean-Up/ibcGetAttachment.jsp?cItemId=17438&fileId=5877708&sitex=10020:22372:US

http://ehs.whoi.edu/ehs/occsafety/EthidiumBromideSafetyProcedure.pdf

Many thanks Dr. Sibnarayan Datta

If the capacity of bondEx EtBr cartridge is

exhausted EtBr breakthrough is

monitored by the connected indicator

cartridge.

Thanks Dr. Maged, I know but we still have a stock of EB in my lab.

we dont pour EB in gel, we use it in buffer then put gel in this solution for dying. this is more safe than when put EB in gel

Hi Arwa,

If you're asking for an alternative to EtBr, Nancy-520 from sigma is for you. Sigma claims, it is both sensitive (for DNA detection) and much safer to use compared to EtBr

Cheers!

Sanjeev

Hi Dr. Arwa,

I recommend using "safe view" dye instead of ethidium bromide.