Any tissue culture protocols available on keratinocytes (epidermal cells) growth and what are some options for growth media?

27 April 2015 0 5K Report

The goal is to observe and identify keratin secretion from tissue culture and components of secretion.

Badges
Science topic

More Michael Amadi's questions See All

Has anyone else received an email regarding payment for citation?

I recently received this email message: "Dear Colleague, I trust you are well! I am writing to propose a mutually beneficial collaboration. I have many research papers that need citations. If...

27 June 2024 9,627 1 View

Do you have a protocol for stimulation of mouse B cells by PWM?

Looking for a protocol for the stimulation of B cells with pokeween mitogen

22 June 2024 4,882 4 View

What is the exact mathematical formulae and the C++ code of EMMS drag model that is used and applied in ANSYS Fluent 2023?

EMMS - Energy Minimization Multi-Scale

18 June 2024 5,857 2 View

Which standards, frameworks or best practices for AI governance do you know?

Frameworks for AI governance The use of artificial intelligence in the company requires its control and monitoring by the company's governing bodies due to legal and regulatory requirements....

17 June 2024 8,956 5 View

Can someone explain the intuition behind, Blue stars are small, but wavelength is big. Red stars are Big but their wavelength is small?

can someone use quantum mechanics to explain the intuition behind this phenomenon?

07 June 2024 9,584 2 View

Can delirium cause brain damage that is similar to damage caused by a stroke?

Elderly patients may develop strokes associated with high blood pressure. Small strokes may result in cognitive dysfunction, confusion, memory lapses and onset of dementia. less frequently and...

07 June 2024 8,723 2 View

How does one get and add a DOI to an existing file upload?

to "Familiar biological, chemical and physical events credibly evolve the Standard Genetic Code", for example?

06 June 2024 5,095 1 View

Which form/type of epigenetic modification is most closely associated with transgenerational effects?

Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...

31 May 2024 4,613 3 View

Help! Alizarin Red not staining?

I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...

28 May 2024 4,068 2 View

How can i get available datasets for Kaduna metropolis at least 2 busy 4-way intersections for research purpose?

I am working on vehicle pedestrian safety at 4-way intersections with traffic signal control lights. I need dataset available for Kaduna metropolis busy 4-way intersection with pedestrian count...

21 May 2024 4,872 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View