Any tips for reducing/eliminating autofluorescence in ovarian tissue?

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EU's affect on Apple's Business?

28 February 2024 6,284 2 View

On average, how many times can you run a Tousimis Samdri-PVT-3D critical point dryer before needing to replace the LCO2 tank?

We do not have a scale to monitor the LCO2 tank weight. Is there any other way to determine when 50% of the net LCO2 has been used? If useful, I am using it to dry bacterial biofilms on glass...

09 March 2021 1,360 0 View

Is it possible to thermally evaporate TiO2 instead of Ti in an oxygen environment?

I am a TA for a vacuums course for sophomores pursuing an engineering physics degree. We are creating a deposition chamber utilizing a turbo and a bell jar for thermal evaporation. We were looking...

05 December 2020 2,173 3 View

What is the state of the art with regards to machine learning in object tracking in video?

I've recently found some time to do some research and find this field particularly interesting. It's been a while since I looked at any research. Just wondering what the latest and greatest is.

29 January 2019 6,444 7 View

Can a logistic regression indicate interactions between many binomial independent variables, as well as differences between subgroups separately?

I am going research looking at several different variables: Dependent variable is binomial (test was ordered or not) Independent variables are also binomial, and there are many (i.e. adult/child,...

09 April 2016 8,996 5 View

Any suggestions for CD86 antibodies for IF with PFA fixed mouse tissue? Can mouse monoclonal antibodies be used on mouse tissue?

IHC-P Tissue IF application

31 March 2016 570 3 View

length of RNAi treatment to achieve gene knockdown in C. elegans?

I am trying to titrate an RNAi feeding of C. elegans. Can anyone tell me how it takes for the worms to experience efficient knockdown?

01 July 2013 135 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View