Hi, I would like to generate a mutant of my protein by mutating its phosphorylated sites (a Thr and a Ser). To which AA can I best change these in order to get the least problems for my protein? I was thinking to change the Thr into a Val and the Ser into an Asn. Does anyone have some experience with this?
Thx a lot
Steven Bergink
Hi Wendy, if you dont want the phosphorylation ala or val (people mostly use ala). If you want phospho-mimicking D or E (Asp or Glu).
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Wendy Toussaint
Hi Steve
I surely dont want phosphorylation so ala or val it is! thx a lot
gr wendy
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