Can the same primer pair , say for instance ITS 1 and ITS 4 generate different molecular weight amplicon after PCR for different fungal species?

Say Fungus A gives 500 bp and Fungus B gives 550 bp is it an absurd result?

29 December 2020 881 3 View

If a certain paper cited is written language other than English, does its interpretation in another authors work become the baseline for it?

A paper not written in English , does it need to be read in its original form before being cited by anyone in the paper that they are writing . Or it is enough to view its interpretation in...

11 October 2020 4,280 8 View

Is it okay to use PCA regression on a time series dataset?

I have a dataset of a binary response variable and 22 predictor variables that have multicollinearity. My goal is to make a predictive model. The data was sampled every 30 seconds over three days....

08 October 2019 5,746 2 View

How to do time series analysis with one/few missing data points?

I have three datasets given to me of images of a conveyor belt taken every 30 seconds over 3 days, but one image was removed/lost in one of them and a few (~10-20) in the others. I calculated the...

02 October 2019 3,893 5 View

Do you think fruit sugar is harmful for human health?

We hear regularly from health organization and experts that we should eat less sugar. As we know that fruit is also valuable source of sugar. So, consumption of more fruit is harmful for human...

22 September 2019 6,270 6 View

How to culture cancer cells the right way?

I am relatively new to cell culturing and I am currently working with several lines of cancer cells, some of them being HeLa, A549, HCT116, etc. I have some very basic doubts regarding the...

03 September 2019 3,003 3 View

How to characterize surface roughness using stereomicroscope pictures when the two samples are different colors (one is much darker)?

I am using the stereomicroscope to generate stacks to capture each plane of the leaf in focus. I am using ImageJ and the plugins SurfCharJ 1q and Fraclac to quantify the leaf roughness and fractal...

04 July 2019 2,282 3 View

Why are XRD images black and white?

XRD images or diffraction spots are black and white. SEM images too. In fact, I think any atomic scale image is black and white. Why are they colorless?

10 April 2019 4,563 3 View

How to extract total protein from strawberry fruit?

I have extracted protein from strawberry by 4% SDS+65mM DTT buffer and found good band in SDS PAGE but when I performed western blot, i didn't observed protein band. What is the reasons? I have...

03 April 2019 7,547 3 View

How to estimate the marginal effects of Bivariate Probit Models in STATA?

Dear All, I am running a Bivariate Probit model in STATA and tried to estimate the marginal effects; however, every time its showing some error message. Could you please help me to run the exact...

25 March 2019 4,637 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View