What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View

How to give port for printed log periodic dipole array antenna in CST?

PLPDA have radiating element on both sides of the substrate. so how to apply port. i have attached the figure below.

03 January 2021 4,005 6 View

I want to design a printed log periodic dipole array antenna on only one side of the substrate. is this acceptable one ?

actually for PLPDA, it is designed on both sides of the substrate for providing 180 degree phase shift between the elements

28 December 2020 3,148 3 View

How to visualize cells seeded on Millicell-HA transwell?

Hi, I am working with Millicell-HA transwell to culture HCE-2 cells. But as the transwell in opaque, not able to visualize the cells. Can someone help me? Thanks in advance.

14 November 2019 3,573 1 View

Can somebody help me with method(s) to analyse and quantify oligonuleotides and protein interactions?

I am looking for an assay design that can help me analyse DNA (oligonucleotides) - protein interactions and provide some means to quantify these interactions. Any help or direction will be much...

14 July 2019 8,475 5 View

What is the calcium concentrations in KSFM and ideal calcium levels for cervical keratinocyte culture?

Hi! I an interested in culturing cervical kerationocytes and calcium levels in media are know to affect their cell growth. I recently obtained two KSFM for the same (one with Calcium and another...

14 May 2019 1,758 3 View

Can we add calcium oxide to graphene oxide?

Suggest any method for the metallisation of graphene oxide

28 January 2019 5,069 3 View

Mixed co culture experiment and MTT assays protocol?

Hello, I am trying to understand a co-culture experiment using different ratios of a cancer cell line and hematopoietic stem cell fraction in a mixed co culture. The ratios range from 1 to 1000...

11 October 2018 697 7 View

How to determine the accurate functional titer of a FLAG tag lentiviral particle?

I have a plasmid of >10 kb that I have made into particles in HEK293T. Before inserting the virus in mice, I would like to determine the functional titer. I have a p24 ELISA kit, but I was...

22 May 2018 3,900 3 View

I am working on ISSR primers. I get bands for some samples and a smear in others. Why?

Hi, I am currently working on ISSR primers for my algal samples. When I did PCR for my samples along with some marine algal specimens, I could observe bands for the marine specimens and a smear...

15 May 2018 877 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Drop weight impact testing machine for composites?

Dear all, I hope everyone is safe and healthy I do have a question please. Where can I find a drop-weight impact testing machine to test fiber reinforced polymer composite plates (CFRP and GFRP)...

17 February 2021 3,820 3 View

Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Where to download DICOM images files to import into MIMICS to build 3D STL models?

I'm working on MDT so in order to build my geometry I need CT or MRI images to import into MIMICS to export my realistic 3D model of arteries, so could anyone introduce me some sites where I can...

14 February 2021 5,003 1 View