Most works use the TNBS assay to quantify free amines on the chitosan when making micelles, to quantify the degree of substitution by the hydrophobic moiety (or any further modifications).
I am running into trouble with chitosan oligosaccharide lactate (https://www.sigmaaldrich.com/GB/en/product/aldrich/523682) and quantifying the free amines with ninhydrin. Is this because of the lactic acid occupying the amines? Is there another reason?
Many thanks in advance,
Swetha
Check https://pubmed.ncbi.nlm.nih.gov/15129991/
https://www.researchgate.net/figure/Slope-and-conversion-based-upon-quantitative-analysis-by-ninhydrin_tbl1_258378393
Hi Dr Swetha Lingamgunta . See the following useful link: https://www.cambridge.org/core/services/aop-cambridge-core/content/view/DE1832340DA8698A6C53E0F5B9A50525/S1946427400364351a.pdf/div-class-title-advantages-and-limitations-of-the-ninhydrin-test-for-analysis-of-historic-wool-fibers-div.pdf
Kindly see also the following good link: https://aata.getty.edu/primo-explore/fulldisplay?docid=GETTY_AATA91037&context=L&vid=AATA&lang=en_US&search_scope=AATA&adaptor=Local%20Search%20Engine&tab=aata&query=creator%2Cexact%2CKerr%2C%20Nancy
Also see the following link: http://obsranking.nahdi.sa/cgi-bin/content/view.php?data=advantages_and_limitations_of_the_ninhydrin_test_for&filetype=pdf&id=14245ee19cacf949176dc47aafe9ad3a
Reactivity of ninhydrin with nucleophiles could be one of the reasons.
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