Fresh spinach leaves were first separated and washed with cold water. Then freeze-dried spinach leaves (50 g) were crushed and extracted with four 25ml portions of acetone and concentrated to about 25 ml and washed with petroleum ether (60-80 °C), (30 ml). Passing pet-ether solution through the column will give green colored extract .Removal of solvent under vacuum gives chlorophyll as a green pigment.

The leaves I'm extracting the chlrophyll from are papaya and avocado leaves.

Use Sephadex LH-20

Extraction in 80 % chilled acetone.

Thank you for sharing your knowledge

The fastest and easiest way is to extract using Dimethylformamide which gives you a very stable extract if kept in the dark without the need to centrifugate. Alan Wellburn wrote a good paper on this subject (1994).

DMF is however toxic so working in a fume hood is advised.

Best of luck