Main theoretical goal is to inhibit formation of growth cone in uncontrolled regenerating peripheral axons. The opposite of SCI studies suggests upregulating RhoA/ROCK2/LIMK pathway will deactivate Cofilin and MLC phosphatase causing collapse of the growth cone. Permanent activation using allosteric agonist of the autoinhibitory Rho associated kinase 2 however will be difficult and likely have bad side effects. Any more specific or generalized suggestions from a medicinal chemistry perspective eg. stimulating CSPGs receptors or preventing microtubule formation in cone?

Thanks!