03 March 2015 5 1K Report

I am doing affinity chromatography for Aprotinin, I am using trypsin affinity column,equilibration and binding of aprotinin ll be done with Tris HCl buffer pH 8.0 and sodium chloride concentration of 0.3M,I just want to know the role of sodium chloride in this affinity chromatography.

why sodium chloride is used at such higher molar concentration,for binding?

if you know any better binding buffer please let me know because i am getting lower binding.

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